Paper Number
PO38 

Session
Poster Session

Title
Rheological characterization of porcine and human subcutaneous-adipose tissue

Presentation Date and Time
October 12, 2022 (Wednesday) 6:30

Track / Room
Poster Session / Riverwalk A

Authors (Click on name to view author profile)

  1. Mitra, Harsa (Purdue University, School of Mechanical Engineering)
  2. Schipper, Eden N. (Purdue University, Weldon School of Biomedical Engineering)
  3. Corder, Ria D. (Purdue University, School of Mechanical Engineering)
  4. Solorio, Luis (Purdue University, Weldon School of Biomedical Engineering)
  5. Ardekani, Arezoo (Purdue University, School of Mechanical Engineering)

Author and Affiliation Lines (in printed abstract book)
Harsa Mitra1, Eden N. Schipper2, Ria D. Corder1, Luis Solorio2 and Arezoo Ardekani1
1School of Mechanical Engineering, Purdue University, West Lafayette, IN 47907; 2Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907

Speaker / Presenter 
Mitra, Harsa

Keywords
experimental methods; biomaterials; rheometry techniques

Text of Abstract
Porcine models are essential in the study of tissue engineering, biomechanics, and medicine. As pigs provide strong clinical relevance to humans, porcine subcutaneous-adipose (SQ) tissue models have been increasingly used to study pharmacokinetics of various biopharmaceuticals through SQ administration. The need for a comprehensive understanding of the rheological behavior of the porcine and human SQ tissues, especially the belly region, becomes necessary. The current study aims to quantify the viscoelastic trends inherent to the porcine and human SQ. Rotational rheology is performed to evaluate SQ viscoelasticity across the porcine neck, belly, breast, and human belly (male and female). The viscoelastic trends are outlaid as a function of frequency, strain amplitude, and relaxation under applied strain. Oscillatory shear tests are performed. To determine the storage and viscous moduli as a function of frequency, frequency-sweep tests are performed at 0.5%. From the amplitude-sweep tests (0.01-0.40% strain), a linear viscoelastic limit is observed at 0.6% strain (0.2% for the human SQ samples) for all the porcine SQ tissues. Large-amplitude oscillatory shear (LAOS) tests are used to illustrate the non-linear stress response at 15% and 40% strain values. Shear-stress relaxation tests are performed at a fixed strain of 40% and 0.01 s rise time. The frequency sweep and amplitude sweep results show a strong fit to a power-law model. The generalized-Maxwell model is used to fit the stress-relaxation behavior.