Paper Number
BF5 

Session
Biomaterials and Bio-fluid Dynamics

Title
Tuning the rheology of collagen-based hydrogels using tannic acid particles

Presentation Date and Time
October 10, 2022 (Monday) 5:05

Track / Room
Track 4 / Michigan AB

Authors (Click on name to view author profile)

  1. Sarker, Prottasha (North Carolina State University, Chemical and Biomolecular Engineering)
  2. Nalband, Danielle M. (North Carolina State University, Joint Department of Biomedical Engineering)
  3. Freytes, Donald O. (North Carolina State University, Joint Department of Biomedical Engineering)
  4. Rojas, Orlando J. (The University of British Columbia, Chemical and Biological Engineering)
  5. Khan, Saad A. (North Carolina State University, Chemical and Biomolecular Engineering)

Author and Affiliation Lines (in printed abstract book)
Prottasha Sarker1, Danielle M. Nalband2, Donald O. Freytes2, Orlando J. Rojas3 and Saad A. Khan1
1Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27606; 2Joint Department of Biomedical Engineering, North Carolina State University, Raleigh, NC 27695; 3Chemical and Biological Engineering, The University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada

Speaker / Presenter 
Sarker, Prottasha

Keywords
experimental methods; biomaterials; gels; microscopy; spectroscopy

Text of Abstract
Collagen-based systems offer significant promise as injectable hydrogels for tissue engineering and regeneration. However, collagen by itself often exhibits poor mechanical strength. The use of plant-derived tannic acid (TA) in combination with collagen offers a unique opportunity to tailor the gel rheology while adding therapeutic functionality from the innate characteristics of the TA. Polyphenolic needle-shaped TA particles or TA solution are mixed with collagen and the temperature- and time- dependent sol-gel transition of the hydrogels monitored as a function of tannic acid particle concentration. Interestingly, particulate tannic acid incorporation generates the largest increase in elastic modulus compared to tannic acid solution at similar concentration. The yield stress determined from the elastic stress method also reveals a similar trend. The behavior of the hydrogels in non-linear regime has also been investigated with large amplitude oscillatory shear (LAOS) measurements. These results are interpreted in terms of the underlying interactions of the tannic acid with collagen using FTIR and other approaches. Hydrogels with tannic acid particles show high cell viability and comparative cellular activity compared to collagen only hydrogel. Development of this completely bio-based hybrid hydrogel system for injectables in tissue regeneration technology can serve as a simple beneficial alternative to difficult implantation scenario.