Paper Number
FP9
Session
Food, Pharmaceuticals & Cosmetics
Title
Rheology and molecular interactions in therapeutic protein solutions
Presentation Date and Time
October 15, 2018 (Monday) 2:45
Track / Room
Track 7 / Plaza II
Authors
- Furst, Eric M. (University of Delaware, Dept. of Chemical and Biomolecular Engineering)
- Woldeyes, Mahlet A. (University of Delaware)
Author and Affiliation Lines
Eric M. Furst1 and Mahlet A. Woldeyes2
1Dept. of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716; 2University of Delaware, Newark, DE
Speaker / Presenter
Furst, Eric M.
Text of Abstract
Improvements in genetic engineering, development, processing, and manufacturing of biotherapeutics have made monoclonal antibodies (mAbs) a leading source of new pharmaceuticals. Currently, there are more than 30 mAbs in clinical trials for autoimmune, oncology, or chronic inflammatory indications. Solution rheology is a key physical property that is often screened during early development and late stage discovery, since downstream processing and manufacturing steps (filtration, pumping, and filling), as well as final administration (including “syringibility”), are substantially affected by the solution viscosity. In this talk, I will focus on the relationship between antibody solution viscosity and molecular interactions of mono- and bispecific molecules. Viscosity and protein interactions are measured using multiple particle tracking (MPT) microrheology and light scattering (LS), respectively. The concentration-, temperature-, and composition-dependence of the solution viscosities will be discussed. The surprising complexity and challenge of establishing clear relations between protein interactions and rheology makes this a rich area for further experimental, theoretical, and computational work.